Uji Aktivitas Antioksidan Ekstrak Metanol Dan Akuades Daun Ganitri (Elaeocarpus ganitrus Roxb.) Dengan Metode Dpph (2,2-Difenil-1-Pikrihidrazil)
Keywords:
Radikal bebas, Antioksidan, Ganitri, DPPH, IC50Abstract
Radikal bebas merupakan atom atau molekul yang mengandung elekton tidak berpasangan sehingga dilakukan penelitian mengenai uji aktivitas antioksidan ekstrak daun ganitri dengan pelarut metanol dan akuades. Tujuan penelitian ini yaitu menentukan kelompok senyawa dan aktivitas antioksidan ekstrak daun ganitri. Hasil uji kelompok senyawa diketahui kedua ekstrak daun ganitri positif mengandung senyawa fenol dan flavonoid. Identifikasi flavonoid dilakukan menggaunkana metode kromatografi dan menghasilkan nilai Rf 0.97 dengan bercak noda berwarna biru kehitaman yang pekat. Uji aktivitas antioksidan dilakukan menggunakan metode DPPH dan didapatkan hasil bahwa kedua ekstrak memiliki aktivitas antioksidan snagat kuat dengan nilai IC50 sebesar 3,213 ppm pada ekstrak metanol dan 4,788 ppm pada ekstrak akuades.
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[4] E. Rachman, “KAJIAN POTENSI DAN PEMANFAATAN JENIS GANITRI ( Elaeocarpus spp. ),” Mitra Hutan Tanam., vol. 7, no. 2, pp. 77–82, 2012.
[5] A. Rohandi and Gunawan, “SEBARAN POPULASI DAN POTENSI TANAMAN GANITRI (Elaeocarpus ganitrus Roxb) DI JAWA TENGAH,” J. ilmu Kehutan., vol. 8, no. 1, pp. 25–33, 2014.
[6] D. Gupta, “Methods for determination of antioxidant capacity : A review,” vol. 6, no. February, 2015.
[7] Kumar, S. Shanmugam, T. Palvannan, and V. M. B. Kumar, “Evaluation of Antioxidant Properties of Elaeocarpus ganitrus Roxb . Leaves,” no. April 2008, pp. 1–6, 2008.
[8] A. Sharma, S. Joshi, and N. Kumar, “Antioxidant and antibacterial properties of leaves of Elaeocarpus sphaericus Roxb. and Pinus wallichiana from Uttarakhand region of India,” Int. J. green Pharm., vol. 9, no. 4, pp. 9–10, 2015.
[9] U. Mayasari and M. T. Laoli, “KARAKTERISASI SIMPLISIA DAN SKRINING FITOKIMIA DAUN JERUK LEMON ( Citrus limon ( L .) Burm . f .),” J. KLOROFIL, vol. 2, no. 1, pp. 7–13, 2018.
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[11] I. Jayashree, D. H. Geetha, and M. Rajeswari, “Evaluation of Anti-Microbial Activity of Elaeocarpus tuberculatus Roxb .,” vol. 16, no. 11, pp. 1726–1731, 2016.
[12] P. E. S. K. Yuda, E. Cahyaningsih, and N. Winariyathi, “SKRINING FITOKIMIA DAN ANALISIS KROMATOGRAFI LAPIS TIPIS EKSTRAK TANAMAN PATIKAN KEBO (Euphorbia hirta L.),” medicamento, vol. 3, no. 2, pp. 61–70, 2017.
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[15] R. Prastiwati, W. S. Rahayu, and D. Hartanti, “perbandingan daya antioksidan ekstrak metanol daun tembakau (Nicotiana tabacum L) dengan rutin terhadap radikal bebas 1,1-diphenil-2-pikrilhidrazil (DPPH),” Pharmacy, vol. 7 (1), no. april 2010, pp. 109–118, 2010.
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[18] D. A. Winahyu, R. C. Purnama, and M. Y. Setiawati, “UJI AKTIVITAS ANTIOKSIDAN PADA EKSTRAK KULIT BUAH NAGA MERAH (Hylocereuspolyrhizus) DENGAN METODE DPPH,” J. Anal. Farm., vol. 4, no. 2, pp. 117–121, 2019.
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[20] F. Setiawan, O. Yunita, and A. Kurniawan, “Uji Aktivitas Antioksidan Ekstrak Etanol Kayu Secang (Caesalpinia sappan) Menggunakan Metode DPPH, ABTS, dan FRAP,” media Pharm. Indones., vol. 2, no. 2, pp. 82–89, 2018.
[21] D. Gupta, “METHODS FOR DETERMINATION OF ANTIOXIDANT CAPACITY: A REVIEW,” Pharm. Sci. Res., vol. 6, no. 2, pp. 546–566, 2015.
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2021-05-27
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Kiromah, N. Z. W., Fitriyati, L., & Husein, S. (2021). Uji Aktivitas Antioksidan Ekstrak Metanol Dan Akuades Daun Ganitri (Elaeocarpus ganitrus Roxb.) Dengan Metode Dpph (2,2-Difenil-1-Pikrihidrazil). Prosiding University Research Colloquium, 79–85. Retrieved from https://repository.urecol.org/index.php/proceeding/article/view/1307
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Copyright (c) 2021 Naelaz Zukhruf Wakhidatul Kiromah, Laeli Fitriyati, Sadam Husein
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