Uji Toksisitas Daun Ketepeng (Cassia Alata L.), Kulit Buah Pisang Ambon (Musa Paradisiaca L. Var Sapientum) dan Kulit Rimpang Kencur (Kaempferia Galanga Linn.) dengan Metode Brine Shrimp Lethality Test (BSLT)

  • Antonius Padua Ratu Sekolah Tinggi Teknologi Industri dan Farmasi Bogor
  • Eko Mugiyanto STIKES Muhammadiyah Pekajangan
Keywords: Artemia salina Leach, BLST, LC50

Abstract

Senyawa antikanker dapat dilakukan skrining dengan metode BSLT.
Metodei ini merupakan metode awal dan salah satu cara yang cepat
serta murah untuk penapisan toksisitas dari ekstrak tanaman dengan
menggunakan hewan laut yaitu larva udang Artemia salina Leach.
Berdasarkan alasan tersebut, maka uji ini sangat tepat digunakan
dalam mengawali penelitian bahan alam. Penelitian ini dilakukan
untuk skrining toksisitas daun ketepeng, kulit buah pisang ambon dan
kulit rimpang kencur dengan metode BSLT sebagai uji awal untuk
mengetahui bioaktivitas. Selain BSLT, juga dilakukan penapisan
fitokimia. Hasil penelitian dengan metode BSLT dari ekstrak etil
asetat daun ketepeng, kulit buah pisang ambon, dan kulit rimpang
kencur menunjukkan LC50 masing-masing sebesar 59,14 ppm,
146,78 ppm, dan kurang dari 10 ppm. Hasil penapisan fitokimia
menunjukkan bahwa ekstrak etil asetat daun ketepeng hanya terdapat
flavonoid, ekstrak etil asetat kulit buah pisang ambon terdapat
flavonid dan terpenoid; dan kulit rimpang kencur terdapat alkaloid
dan terpenoid. Ekstrak etil asetat kulit rimpang kencur menunjukan
aktivitas tertinggi dan pontesial sebagai antikanker, maka perlu
dilakukan isolasi dan identifikasi sehingga diperoleh senyawa aktif.
Anticancer compounds can be screened by the BSLT method. This
method is an early method and one of the quickest and cheapest ways
to screen the toxicity of plant extract using marine animals shrimp
larvae Artemia salina Leach. Based on these reasons, this research
was very appropriate to use in initiating the research of natural
materials. This research was conducted for toxicity screening of
ketepeng leaf, banana peel and kencur rhizome skin by BSLT method
as preliminary test to know bioactivity. Besides BSLT, phytochemical
screening is also performed. The result of BSLT method from the
extract of ethyl acetate of ketepeng leaf, banana peel, and kencur
rhizome skin showed LC50 of 59.14 ppm, 146,78 ppm and less than
10 ppm, respectively. The results of phytochemical screening showed
that the extract of ethyl acetate of ketepeng leaf contained only
flavonoid, ethyl acetate extract of banana peel contained flavonoid
and terpenoid; and kencur rhizome skin kencur contain alkaloid and
terpenoid. The ethyl acetate extract of the skin of kencur rhizome skin
shows the highest activity and pontesial as anticancer, it is necessary
to isolate and identify so that the active compound is obtained.

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Published
2018-02-21